Fast Fatty Acid Analysis by Core-Shell Reversed-Phase Liquid Chromatography Coupled to Evaporative Light-Scattering Detector

Florence Hubert (1) , Céline Loiseau (1) , Françoise Ergan (1) , Gaëlle Pencréac’h (1) , Laurent Poisson (1)

1 MMS - Mer, molécules et santé EA 2160

A high-performance liquid chromatography analysis method with an evapor-ative light-scattering detector has been developed for the separation and quantitative analysis of free fatty acids in biological matrices. Core-shell reversed phase high-performance liquid chromatography separation of 10 free fatty acids is achieved within 10.5 min using a methanol/water (0.05% trifluo-roacetic acid) eluent gradient. After optimization, the drift tube and nebuliza-tion temperature of the evaporative light-scattering detector was set at 35˚C, nitrogen flow-rate at 1.1 standard liter per minute and column temperature at 25˚C. All calibration curves showed good regression (r 2 > 0.9975). A validation procedure following the International Conference on Harmonisation guidelines was implemented to certify the method. Relative standard deviations did not exceed 1.5% and 4.25% for repeatability and reproducibility respectively.

Mots clés: Free Fatty Acids; HPLC; Evaporative Light-Scattering Detector; Core-Shell Technology; Method Validation

Domaines: Sciences du Vivant [q-bio]; Chimie

Florence Hubert, Céline Loiseau, Françoise Ergan, Gaëlle Pencréac’h, Laurent Poisson. Fast Fatty Acid Analysis by Core-Shell Reversed-Phase Liquid Chromatography Coupled to Evaporative Light-Scattering Detector. Food and Nutrition Sciences, 2017, 08 (12), pp.1051 - 1062. ⟨10.4236/fns.2017.812077⟩. ⟨hal-01875845⟩