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Fast Fatty Acid Analysis by Core-Shell Reversed-Phase Liquid Chromatography Coupled to Evaporative Light-Scattering Detector

Abstract : A high-performance liquid chromatography analysis method with an evapor-ative light-scattering detector has been developed for the separation and quantitative analysis of free fatty acids in biological matrices. Core-shell reversed phase high-performance liquid chromatography separation of 10 free fatty acids is achieved within 10.5 min using a methanol/water (0.05% trifluo-roacetic acid) eluent gradient. After optimization, the drift tube and nebuliza-tion temperature of the evaporative light-scattering detector was set at 35˚C, nitrogen flow-rate at 1.1 standard liter per minute and column temperature at 25˚C. All calibration curves showed good regression (r 2 > 0.9975). A validation procedure following the International Conference on Harmonisation guidelines was implemented to certify the method. Relative standard deviations did not exceed 1.5% and 4.25% for repeatability and reproducibility respectively.
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https://hal-univ-lemans.archives-ouvertes.fr/hal-01875845
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Soumis le : lundi 17 septembre 2018 - 20:55:29
Dernière modification le : jeudi 26 mars 2020 - 09:38:31

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Florence Hubert, Céline Loiseau, Françoise Ergan, Gaëlle Pencréac’h, Laurent Poisson. Fast Fatty Acid Analysis by Core-Shell Reversed-Phase Liquid Chromatography Coupled to Evaporative Light-Scattering Detector. Food and Nutrition Sciences, Scientific Research, 2017, 08 (12), pp.1051 - 1062. ⟨10.4236/fns.2017.812077⟩. ⟨hal-01875845⟩

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