DNA Binding Specificity and Cleavage Activity of Pacmmar TransposaseRID C-6465-2009 - Le Mans Université Accéder directement au contenu
Article Dans Une Revue Biochemistry Année : 2009

DNA Binding Specificity and Cleavage Activity of Pacmmar TransposaseRID C-6465-2009

Résumé

Mariner-like elements (MLEs) are members of the Tc1/mariner superfamily of transposable elements which transpose by a "cut and paste" mechanism. Most of the MLEs characterized to date are transpositionally inactive due to the accumulation of mutations in their transposase gene. Here, we report the biochemical study of two copies of the Pacmmar element (Pacmmar1.1 and Pacmmar1.2), isolated from the coastal crab Pachygrapsus marmoratus. These two copies present an open reading frame encoding a putative active transposase. Using an in vitro transposition assay, we show that Pacmmar transposases are unable to perform by themselves the transposition reaction. However, we demonstrate by an electrophoretic mobility shift assay that both transposases bind specifically to the inverted terminal repeat of the Pacmmar element. Moreover, an in vitro cleavage assay showed that both transposases have the capacity to cleave the transposon. The in vitro cleavage activity of Pacmmar transposases appears imprecise, suggesting the requirement of specific host factors or the presence of mutations which have modified the cleavage specificity of the enzyme.
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Dates et versions

hal-00617476 , version 1 (29-08-2011)

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Laurence Delauriere, Benoit Chenais, Elisabeth Pradier, Yann Hardivillier, Sylvaine Renault, et al.. DNA Binding Specificity and Cleavage Activity of Pacmmar TransposaseRID C-6465-2009. Biochemistry, 2009, 48 (30), pp.7279-7286. ⟨10.1021/bi900609v⟩. ⟨hal-00617476⟩
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