Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Transcriptomic Response of Breast Cancer Cells MDA-MB-231 to Docosahexaenoic Acid: Downregulation of Lipid and Cholesterol Metabolism Genes and Upregulation of Genes of the Pro-Apoptotic ER-Stress Pathway

Abstract : Despite considerable e↵orts in prevention and therapy, breast cancer remains a major public health concern worldwide. Numerous studies using breast cancer cell lines have shown the antiproliferative and pro-apoptotic e↵ects of docosahexaenoic acid (DHA). Some studies have also demonstrated the inhibitory e↵ect of DHA on the migration and invasion of breast cancer cells, making DHA a potential anti-metastatic agent. Thus, DHA has shown its potential as a chemotherapeutic adjuvant. However, the molecular mechanisms triggering DHA e↵ects remain unclear, and the aim of this study was to provide a transcriptomic basis for further cellular and molecular investigations. Therefore, MDA-MB-231 cells were treated with 100 µM DHA for 12 h or 24 h before RNA-seq analysis. The results show the great impact of DHA-treatment on the transcriptome, especially after 24 h of treatment. The impact of DHA is particularly visible in genes involved in the cholesterol biosynthesis pathway that is strongly downregulated, and the endoplasmic reticulum (ER)-stress response that is, conversely, upregulated. This ER-stress and unfolded protein response could explain the pro-apoptotic e↵ect of DHA. The expression of genes related to migration and invasion (especially SERPINE1, PLAT, and MMP11) is also impacted by DHA. In conclusion, this transcriptomic analysis supports the antiproliferative, pro-apoptotic and anti-invasive e↵ects of DHA, and provides new avenues for understanding its molecular mechanisms.
Liste complète des métadonnées

Littérature citée [107 références]  Voir  Masquer  Télécharger

https://hal-univ-lemans.archives-ouvertes.fr/hal-02893193
Contributeur : Benoît Chénais <>
Soumis le : mercredi 8 juillet 2020 - 09:56:10
Dernière modification le : mardi 21 juillet 2020 - 16:44:57

Fichier

Chenais_2020_ijerph.pdf
Publication financée par une institution

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Citation

Benoît Chénais, Marine Cornec, Solenne Dumont, Justine Marchand, Vincent Blanckaert. Transcriptomic Response of Breast Cancer Cells MDA-MB-231 to Docosahexaenoic Acid: Downregulation of Lipid and Cholesterol Metabolism Genes and Upregulation of Genes of the Pro-Apoptotic ER-Stress Pathway. International Journal of Environmental Research and Public Health, MDPI, 2020, ⟨10.3390/ijerph17103746⟩. ⟨hal-02893193⟩

Partager

Métriques

Consultations de la notice

34

Téléchargements de fichiers

62